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Application of a chip-based capillary electrophoresis system to enable simple PCR detection of fish species

Project Code: Q01069

27/07/2010

Campden BRI
Dooley, J ; Garrett, S; Sage, H; Clark, M; Brown, H

The common name of certain fish and fish products is required to be given when they are sold. Due to differences in market value, fishing quota restrictions and emergence of new farmed fish species, there is significant incentive for species mislabelling. Reliable fish identification methods are therefore needed. The identification of fish species becomes more difficult once it has been processed and morphological characteristics are no longer visible. Methods determining differences in proteins have traditionally been used for fish species identification. However, these methods are not always reliable when used on highly processed foods as the proteins become denatured and only a limited number of fish species immunoassays have been developed.
DNA based methods have therefore become increasingly appealing as an approach to fish species identification as DNA remains detectable in all but the most heavily processed samples. Species identification is achieved by detection of differences in the DNA sequence between fish.

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