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The serodiagnosis of infections caused by verocytoxin-producing Escherichia coli based on salivary antibiotics to E. coli lipopolysaccharides

Project Code: B11008

01/11/2012

Laboratory of Enteric Pathogens, Centre for Infections, Department of Gastrointestinal Infections, Specialist and Reference Microbiology Division
Chart, H ;
HPA Colindale
Smith, H

The overall aims of the project were to determine the levels of salivary antibodies produced to lipopolysaccharide expressed by Verocytotoxin-producing Escherichia coli (VTEC), and in particular VTEC O157 LPS, during the pathogenesis of infections with these bacteria. Also to elucidate the kinetics of salivary antibody production and establish when antibody expression was maximal and the duration of antibody secretion. It was also intended to determine the stability of antibodies in saliva, during transportation to the Laboratory of Enteric Pathogens, at ambient temperature.

From the study it was concluded that patients infected with E. coli O157, may have salivary antibodies to these organisms. The commercial sampling kits were shown to be suitable for collecting samples of saliva and that antibodies in saliva were stable during transport to the testing laboratory. The tests employed were able to detect antibodies in saliva.

Final report

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