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A critical review of methods for distinguishing infectious and non-infectious norovirus
Project Code: FS101036;
- Knight, Angus; Li, Dan; Uyttendeale, Mieke; Jaykus, Lee-Ann (2012), A critical review of methods for detecting human noroviruses and protecting their infectivity, Critical Reviews in Microbiology, (online publication) doi:10.3109/1040841X.2012.709820
Leatherhead Food International
This review aimed to identify methods used to detect norovirus in food, the environment and in clinical samples. The study assessed the potential of these methods to distinguish infectious and non-infectious norovirus, focusing particularly on approaches capable of assessing whether norovirus capsid or RNA can be used as a guide to the likelihood of the virus infecting people.
Comparison of data in this review was complicated by the wide range of different viruses and experimental conditions used in different studies. In particular, experimental conditions need to be controlled carfully to allow comparison of studies. Particular attention should be paid to important variables including treatment type and conditions, virus concentration, matrix composition, enzyme activity, and potential RT-qPCR (Reverse Transcription Polymerase Chain Reaction) inhibition. Nevertheless, this review has identified gaps in our knowledge regarding the detection of infectious human norovirus.
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