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The analysis of urine samples for biomarkers of exposure to Fusarium mycotoxins
Project Code: C03044;
- Turner, P.C., White, K.L.M., Burley, V.J., Hopton, R.P., Rajendram, A., Fisher, J., Cade, J.E. & Wild, C.P. (2010). A comparison of deoxynivalenol intake and urinary deoxynivalenol in UK adults. Biomarkers, Published on-line doi:10.3109/1354750X.2010.495787,1-10
- Turner, P.C., Hopton, R.P., White, K.L.M., Fisher, J., Cade,J.L., & Wild, C.P. (2011) Assessment of deoxynivalenol metabolite profiles in UK adults. Food Chem Toxicol. 49(1), 132-135 doi: 10.1016/j.fct.2010.10.007
University of Leeds
Turner, P ; Cade, J; Wild, C
Phase 1 of the study showed that cereal intake was significantly associated with urinary deoxynivalenol (DON), assessed using an LC-MS method. A method was also developed to measure urinary fumonisins with a level of detection at 0.02 ng/ml, however, no urinary fumonisins were detected in the NDNS urine samples.
Phase 2 of the study showed that urinary DON was significantly associated with DON intake and the variation in urinary DON correlated to DON intake. Cereal consumption, in particular bread, significantly contributed to the levels of urinary DON observed in UK adults. The transfer of DON to urine was estimated to be 72.3% and it was cleared from urine within a 24 to 48 hour period.
Using the mean estimated DON intake from phase 1 (0.2 μg/kg bw/day and the estimated DON transfer to urine of 72.3%, 2/300 were predicted to exceed the recommended Tolerable Daily Intake (TDI) for DON. It should be noted that these individuals were a stratified sample based on cereal consumption.
Overall the data indicate that urinary DON was sensitive enough to measure DON exposure at the individual level in the UK population.
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