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An investigation into the use of Mass Spectrometry to quantify specific DNA fragments generated from GMO's

Project Code: G01024

30/06/2005

RHM Technology
Wiseman, G ; Oldham, N

The purpose of this project was to investigate the many challenges involved with the isolation and detection of specific DNA sequences without recourse to PCR. Initial evidence has been obtained using specific DNA sequences cut from genomic soya DNA that have been captured and concentrated using a combination of biochemical techniques.

The project has investigated a novel method of characterising the captured DNA based upon its precise molecular weight using Electrospay Ionisation Mass Spectrometry (ESIMS). The advances described open the way to the detection and characterisation of specific DNA fragments present either in GMOs or other important food analytes without the need to amplify the DNA first using PCR. These innovations have important implications for the future accurate quantification of food analytes. The methods described are the first steps in generating innovative technical solutions that would benefit manufacturers, retailers, enforcement bodies and customers alike in their challenges
regarding the correct description of food.

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