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Dietary nitrate consumption: an investigation of biomarkers of DNA & protein damage in humans

Project Code: C01013

31/01/2003

Kings College London
Rice-Evans, C ;
Department of Medicine, Royal Free and University College Medical School
Moore, K

AIMS

The major aims of this study are:

PHASE I

a. To examine the relationship between nitrate intake from a commercially available glasshouse grown round green lettuce (British produce) in the diet with beneficial effects on platelet function and biomarkers of adverse effects of RNS, namely, deamination of DNA bases derived from white blood cell DNA, and protein modification through detection of 3-nitrotyrosine in blood plasma,

b. To examine the extent to which nitrate intake from vegetables contributes to levels of nitrate and nitrite in blood plasma, saliva and urine, over and above that derived from endogenous nitric oxide production,

PHASE II

c. To examine in individuals undergoing treatment for gastric dysfunction, the influence of dietary nitrate on levels of nitrate and nitrite and the formation of 3-nitrotyrosine apart from plasma and urine in gastric juice.

This aim was further modified to include:

To study the effect of dietary nitrate supplementation on nitrate metabolism and production of biomarkers of reactive nitrogen species formation with respect to alterations in gastric pH, in 18 normal healthy volunteers and 12 patients suffering from Helicobacter pylori infection over a three-day period.

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