View Report Details
Investigation of the putative neurotoxic effect of cockle extracts using extracellular field potential recordings in hippocampal slices
Project Code: B16004
An in vitro assay was developed to test for potential neurotoxic effects of compounds and substances. Extracellular recording techniques were used to record the effects of known and putative neurotoxins on field excitatory postsynaptic potentials (fEPSP) in in vitro hippocampal brain slice preparations. The toxins; Brevetoxin, Domoic acid and Saxiotoxin dihydrochloride all induced concentration-dependent suppression of fEPSPs, whereas Okadaic acid was without significant effect. The hippocampal fEPSP assay demonstrated a highly sensitive and reproducible means of establishing the toxic potential of cockle extracts, with each of the four known neurotoxins producing a specific ‘signature’ allowing the differentiation of specific toxins at high concentrations. The effects of a toxic cockle extract (positive extract) along with a control non-toxic extract (negative extract) were subsequently tested. The concentration range to be tested was first established by testing the effects of the solvent/carrier 1% TWEEN-60, used for this study. The positive cockle extract was without significant effect at a concentration of 1:5000 compared to control. Thus, supplementary data was obtained at higher concentrations. At higher concentrations, the positive cockle extract was still without significant effect compared to control and negative cockle extract. We conclude from this data that the cockle extract has no obvious toxicity in this assay. However, these data do not discount the possibility that the toxin may have as yet an undefined neurotoxic action, nor does this data discount the possibility that the toxin acts at peripheral neurones as a neuromuscular blocker-like compound. Further studies are required to test this possibility.
Some of the files on this site may be in a format that your computer can't read. However, you can download Readers and Viewers for the following document types below: